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A guideline for sample preparation in modern tree-ring stable isotope research

Authors
/persons/resource/kscholla

Schollaen,  Karina
5.2 Climate Dynamics and Landscape Evolution, 5.0 Geoarchives, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

Baschek,  Heiko
External Organizations;

/persons/resource/heinrich

Heinrich,  Ingo
5.2 Climate Dynamics and Landscape Evolution, 5.0 Geoarchives, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

/persons/resource/wraenci

Slotta,  Franziska
5.2 Climate Dynamics and Landscape Evolution, 5.0 Geoarchives, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

/persons/resource/mpauly

Pauly,  Maren
5.2 Climate Dynamics and Landscape Evolution, 5.0 Geoarchives, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

/persons/resource/ghelle

Helle,  G.
5.2 Climate Dynamics and Landscape Evolution, 5.0 Geoarchives, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

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Citation

Schollaen, K., Baschek, H., Heinrich, I., Slotta, F., Pauly, M., Helle, G. (2017): A guideline for sample preparation in modern tree-ring stable isotope research. - Dendrochronologia, 44, 133-145.
https://doi.org/10.1016/j.dendro.2017.05.002


Cite as: https://gfzpublic.gfz-potsdam.de/pubman/item/item_2268913
Abstract
The comprehensive procedure of wood sample preparation, including tree-ring dissection, cellulose extraction, homogenization and packing for stable isotope analysis, is labour intensive and time consuming. Based on a brief compilation of existing methods, we present a methodological approach from pre-analyses considerations to wood sample preparation, semi-automated chemical extraction of cellulose from tree-ring cross-sections, and tree-ring dissection for stable isotope ratio mass spectrometry: the Cross-Section Extraction and Dissection (CSED) guideline. Following the CSED guideline can considerably increase efficiency of tree-ring stable isotope measurement compared to classical methods <ABS-P>We introduce a user-friendly device for cellulose extraction, allowing simultaneous treatment of wood cross-sections of a total length of 180 cm (equivalent to 6 increment cores of 30 cm length) and thickness of 0.6–2.0 mm. After cellulose extraction, tree-ring structures of 10 tree species (coniferous and angiosperm wood) with different wood growth rates and tree-ring boundaries, largely remained well identifiable. Further, we demonstrate that tree rings from cellulose cross-sections can be dissected at annual to intra-seasonal resolution, utilizing simple manual devices as well as sophisticated UV-laser microdissection microscopes in a way that sample homogenization is no longer necessary in most cases. We investigate seasonal precipitation signals in high-resolution intra-annual δ18O cellulose values from African baobab, performed by using UV-laser microdissection microscopes.