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Journal Article

A high yield cellulose extraction system for small whole wood samples and dual measurement of carbon and oxygen stable isotopes

Authors

Andreu-Hayles,  Laia
External Organizations;

Levesque,  Mathieu
External Organizations;

Martin-Benito,  Dario
External Organizations;

Huang,  Wei
External Organizations;

Harris,  Ryan
External Organizations;

Oelkers,  Rose
External Organizations;

Leland,  Caroline
External Organizations;

Martin-Fernández,  Javier
External Organizations;

Anchukaitis,  Kevin J.
External Organizations;

/persons/resource/ghelle

Helle,  G.
4.3 Climate Dynamics and Landscape Evolution, 4.0 Geosystems, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

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Fulltext (public)

3571898.pdf
(Publisher version), 15MB

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Citation

Andreu-Hayles, L., Levesque, M., Martin-Benito, D., Huang, W., Harris, R., Oelkers, R., Leland, C., Martin-Fernández, J., Anchukaitis, K. J., Helle, G. (2019): A high yield cellulose extraction system for small whole wood samples and dual measurement of carbon and oxygen stable isotopes. - Chemical Geology, 504, 53-65.
https://doi.org/10.1016/j.chemgeo.2018.09.007


Cite as: https://gfzpublic.gfz-potsdam.de/pubman/item/item_3571898
Abstract
This paper describes devices to extract α-cellulose from small whole wood samples developed at the Lamont-Doherty Earth Observatory Tree-Ring Lab and explains the procedures for chemical extractions and for the dual analysis of carbon (δ13C) and oxygen (δ18O) stable isotopes. Here, we provide the necessary steps and guidelines for constructing a cellulose extraction system for small amounts of wood and leaves. The system allows the simultaneous extraction of cellulose from 150 samples by means of in-house filter tubes, where chemicals used for the cellulose extraction are exchanged and eliminated in batches. This new implementation diminishes the processing time, minimizes physical sample manipulation and potential errors, increases sample throughput, and reduces the amount of chemicals and analytic costs. We also describe the dual measurement of δ13C and δ18O ratios in tree-ring cellulose using high-temperature pyrolysis in a High Temperature Conversion Elemental Analyzer (TC/EA) interfaced with a Thermo Delta V plus mass spectrometer.