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Abstract:
The Nostoc sp. strain CCCryo 231-06 is a cyanobacterial strain capable of surviving
under extreme conditions and thus is of great interest for the astrobiology
community. The knowledge of its complete genome sequence would serve as a
guide for further studies. However, a major concern has been placed on the effects
of contamination on the quality of sequencing data without a reference
genome. Here,we report the use ofmicrofluidic technology combinedwith single
cell sequencing and de novo assembly tominimize the contamination and recover
the complete genome of the Nostoc strain CCCryo 231-06 with high quality.
100% of the whole genome was recovered with all contaminants removed and
a strongly supported phylogenetic tree. The data reported can be useful for
comparative genomics for phylogenetic and taxonomic studies. The method
used in this work can be applied to studies that require high-quality assemblies
of genomes of unknown microorganisms.
