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Journal Article

Whole genome sequencing of cyanobacterium Nostoc sp. CCCryo 231-06 using microfluidic single cell technology

Authors

Liu,  Yuguang
External Organizations;

Jeraldo,  Patricio
External Organizations;

Herbert,  William
External Organizations;

McDonough,  Samantha
External Organizations;

Eckloff,  Bruce
External Organizations;

/persons/resource/dirksm

Schulze-Makuch,  Dirk
3.7 Geomicrobiology, 3.0 Geochemistry, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

de Vera,  Jean-Pierre
External Organizations;

Cockell,  Charles
External Organizations;

Leya,  Thomas
External Organizations;

Baqué,  Mickael
External Organizations;

Jen,  Jin
External Organizations;

Walther-Antonio,  Marina
External Organizations;

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Fulltext (public)

5012157.pdf
(Publisher version), 3MB

Supplementary Material (public)
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Citation

Liu, Y., Jeraldo, P., Herbert, W., McDonough, S., Eckloff, B., Schulze-Makuch, D., de Vera, J.-P., Cockell, C., Leya, T., Baqué, M., Jen, J., Walther-Antonio, M. (2022): Whole genome sequencing of cyanobacterium Nostoc sp. CCCryo 231-06 using microfluidic single cell technology. - iScience, 25, 5, 104291.
https://doi.org/10.1016/j.isci.2022.104291


Cite as: https://gfzpublic.gfz-potsdam.de/pubman/item/item_5012157
Abstract
The Nostoc sp. strain CCCryo 231-06 is a cyanobacterial strain capable of surviving under extreme conditions and thus is of great interest for the astrobiology community. The knowledge of its complete genome sequence would serve as a guide for further studies. However, a major concern has been placed on the effects of contamination on the quality of sequencing data without a reference genome. Here,we report the use ofmicrofluidic technology combinedwith single cell sequencing and de novo assembly tominimize the contamination and recover the complete genome of the Nostoc strain CCCryo 231-06 with high quality. 100% of the whole genome was recovered with all contaminants removed and a strongly supported phylogenetic tree. The data reported can be useful for comparative genomics for phylogenetic and taxonomic studies. The method used in this work can be applied to studies that require high-quality assemblies of genomes of unknown microorganisms.