date: 2023-10-25T14:00:08Z
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 Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches
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subject: DOI: 10.1093/femsec/fiad119 FEMS Microbiology Ecology, 99, 11, 3-10-2023. Abstract: The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting.
dc:creator: Jaarsma Ate H., Sipes Katie, Zervas Athanasios, Jiménez Francisco Campuzano, Ellegaard-Jensen Lea, Thøgersen Mariane S., Stougaard Peter, Benning Liane G., Tranter Martyn, Anesio Alexandre M.
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title: 

 Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches
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dc:title: 

 Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches
modified: 2023-10-25T14:00:08Z
cp:subject: DOI: 10.1093/femsec/fiad119 FEMS Microbiology Ecology, 99, 11, 3-10-2023. Abstract: The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting.
pdf:docinfo:subject: DOI: 10.1093/femsec/fiad119 FEMS Microbiology Ecology, 99, 11, 3-10-2023. Abstract: The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting.
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pdf:docinfo:creator: Jaarsma Ate H., Sipes Katie, Zervas Athanasios, Jiménez Francisco Campuzano, Ellegaard-Jensen Lea, Thøgersen Mariane S., Stougaard Peter, Benning Liane G., Tranter Martyn, Anesio Alexandre M.
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creator: Jaarsma Ate H., Sipes Katie, Zervas Athanasios, Jiménez Francisco Campuzano, Ellegaard-Jensen Lea, Thøgersen Mariane S., Stougaard Peter, Benning Liane G., Tranter Martyn, Anesio Alexandre M.
meta:author: Jaarsma Ate H., Sipes Katie, Zervas Athanasios, Jiménez Francisco Campuzano, Ellegaard-Jensen Lea, Thøgersen Mariane S., Stougaard Peter, Benning Liane G., Tranter Martyn, Anesio Alexandre M.
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Author: Jaarsma Ate H., Sipes Katie, Zervas Athanasios, Jiménez Francisco Campuzano, Ellegaard-Jensen Lea, Thøgersen Mariane S., Stougaard Peter, Benning Liane G., Tranter Martyn, Anesio Alexandre M.
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pdf:docinfo:producer: Acrobat Distiller 23.0 (Windows); modified using iTextSharp 5.5.10 ©2000-2016 iText Group NV (AGPL-version)
pdf:docinfo:created: 2023-10-17T03:22:24Z
doi: 10.1093/femsec/fiad119