date: 2024-04-24T12:07:27Z pdf:PDFVersion: 1.6 pdf:docinfo:title: Persistent microbial communities in hyperarid subsurface habitats of the Atacama Desert: Insights from intracellular DNA analysis xmp:CreatorTool: Servigistics Arbortext Advanced Print Publisher 11.1.4546/W-x64 access_permission:can_print_degraded: true subject: DOI: 10.1093/pnasnexus/pgae123; PNAS Nexus, 3, 4, 2024-4-23.; Abstract: Desert environments constitute one of the largest and yet most fragile ecosystems on Earth. Under the absence of regular precipitation, microorganisms are the main ecological component mediating nutrient fluxes by using soil components, like minerals and salts, and atmospheric gases as a source for energy and water. While most of the previous studies on microbial ecology of desert environments have focused on surface environments, little is known about microbial life in deeper sediment layers. Our study is extending the limited knowledge about microbial communities within the deeper subsurface of the hyperarid core of the Atacama Desert. By employing intracellular DNA extraction and subsequent 16S rRNA sequencing of samples collected from a soil pit in the Yungay region of the Atacama Desert, we unveiled a potentially viable microbial subsurface community residing at depths down to 4.20?m. In the upper 80?cm of t dc:format: application/pdf; version=1.6 pdf:docinfo:creator_tool: Servigistics Arbortext Advanced Print Publisher 11.1.4546/W-x64 access_permission:fill_in_form: true pdf:encrypted: false dc:title: Persistent microbial communities in hyperarid subsurface habitats of the Atacama Desert: Insights from intracellular DNA analysis modified: 2024-04-24T12:07:27Z cp:subject: DOI: 10.1093/pnasnexus/pgae123; PNAS Nexus, 3, 4, 2024-4-23.; Abstract: Desert environments constitute one of the largest and yet most fragile ecosystems on Earth. Under the absence of regular precipitation, microorganisms are the main ecological component mediating nutrient fluxes by using soil components, like minerals and salts, and atmospheric gases as a source for energy and water. While most of the previous studies on microbial ecology of desert environments have focused on surface environments, little is known about microbial life in deeper sediment layers. Our study is extending the limited knowledge about microbial communities within the deeper subsurface of the hyperarid core of the Atacama Desert. By employing intracellular DNA extraction and subsequent 16S rRNA sequencing of samples collected from a soil pit in the Yungay region of the Atacama Desert, we unveiled a potentially viable microbial subsurface community residing at depths down to 4.20?m. In the upper 80?cm of t pdf:docinfo:subject: DOI: 10.1093/pnasnexus/pgae123; PNAS Nexus, 3, 4, 2024-4-23.; Abstract: Desert environments constitute one of the largest and yet most fragile ecosystems on Earth. Under the absence of regular precipitation, microorganisms are the main ecological component mediating nutrient fluxes by using soil components, like minerals and salts, and atmospheric gases as a source for energy and water. While most of the previous studies on microbial ecology of desert environments have focused on surface environments, little is known about microbial life in deeper sediment layers. Our study is extending the limited knowledge about microbial communities within the deeper subsurface of the hyperarid core of the Atacama Desert. By employing intracellular DNA extraction and subsequent 16S rRNA sequencing of samples collected from a soil pit in the Yungay region of the Atacama Desert, we unveiled a potentially viable microbial subsurface community residing at depths down to 4.20?m. In the upper 80?cm of t pdf:docinfo:creator: Lucas Horstmann meta:author: Daniel Lipus meta:creation-date: 2024-04-17T02:38:45Z created: Wed Apr 17 04:38:45 CEST 2024 access_permission:extract_for_accessibility: true Creation-Date: 2024-04-17T02:38:45Z Author: Daniel Lipus producer: PDFlib+PDI 9.0.7p3 (C++/Win64); modified using iTextSharp 4.1.6 by 1T3XT pdf:docinfo:producer: PDFlib+PDI 9.0.7p3 (C++/Win64); modified using iTextSharp 4.1.6 by 1T3XT pdf:docinfo:custom:EPSprocessor: PStill version 1.84.42 dc:description: DOI: 10.1093/pnasnexus/pgae123; PNAS Nexus, 3, 4, 2024-4-23.; Abstract: Desert environments constitute one of the largest and yet most fragile ecosystems on Earth. Under the absence of regular precipitation, microorganisms are the main ecological component mediating nutrient fluxes by using soil components, like minerals and salts, and atmospheric gases as a source for energy and water. While most of the previous studies on microbial ecology of desert environments have focused on surface environments, little is known about microbial life in deeper sediment layers. Our study is extending the limited knowledge about microbial communities within the deeper subsurface of the hyperarid core of the Atacama Desert. By employing intracellular DNA extraction and subsequent 16S rRNA sequencing of samples collected from a soil pit in the Yungay region of the Atacama Desert, we unveiled a potentially viable microbial subsurface community residing at depths down to 4.20?m. In the upper 80?cm of t Keywords: Atacama; hyperarid; desert soil; subsurface; microbial community access_permission:modify_annotations: true dc:creator: Daniel Lipus description: DOI: 10.1093/pnasnexus/pgae123; PNAS Nexus, 3, 4, 2024-4-23.; Abstract: Desert environments constitute one of the largest and yet most fragile ecosystems on Earth. Under the absence of regular precipitation, microorganisms are the main ecological component mediating nutrient fluxes by using soil components, like minerals and salts, and atmospheric gases as a source for energy and water. While most of the previous studies on microbial ecology of desert environments have focused on surface environments, little is known about microbial life in deeper sediment layers. Our study is extending the limited knowledge about microbial communities within the deeper subsurface of the hyperarid core of the Atacama Desert. By employing intracellular DNA extraction and subsequent 16S rRNA sequencing of samples collected from a soil pit in the Yungay region of the Atacama Desert, we unveiled a potentially viable microbial subsurface community residing at depths down to 4.20?m. In the upper 80?cm of t dcterms:created: 2024-04-17T02:38:45Z Last-Modified: 2024-04-24T12:07:27Z dcterms:modified: 2024-04-24T12:07:27Z title: Persistent microbial communities in hyperarid subsurface habitats of the Atacama Desert: Insights from intracellular DNA analysis xmpMM:DocumentID: uuid:4C8EFF07-36EF-23D8-3B0F-1BA3B0AE0712 Last-Save-Date: 2024-04-24T12:07:27Z pdf:docinfo:keywords: Atacama; hyperarid; desert soil; subsurface; microbial community pdf:docinfo:modified: 2024-04-24T12:07:27Z meta:save-date: 2024-04-24T12:07:27Z Content-Type: application/pdf X-Parsed-By: org.apache.tika.parser.DefaultParser creator: Daniel Lipus EPSprocessor: PStill version 1.84.42 dc:subject: Atacama; hyperarid; desert soil; subsurface; microbial community access_permission:assemble_document: true xmpTPg:NPages: 12 access_permission:extract_content: true access_permission:can_print: true meta:keyword: Atacama; hyperarid; desert soil; subsurface; microbial community access_permission:can_modify: true pdf:docinfo:created: 2024-04-17T02:38:45Z